Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.
Identifieur interne : 003549 ( Main/Exploration ); précédent : 003548; suivant : 003550Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.
Auteurs : Nicolas Brüggemann [Allemagne] ; Rudolf Meier ; Dominik Steigner ; Ina Zimmer ; Sandrine Louis ; Jörg-Peter SchnitzlerSource :
- The New phytologist [ 1469-8137 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Aérobiose (effets des radiations), Bactéries (métabolisme), Dioxyde de carbone (métabolisme), Isotopes du carbone (MeSH), Lumière (MeSH), Marquage isotopique (MeSH), Méthane (métabolisme), Photosynthèse (effets des radiations), Populus (effets des radiations), Populus (métabolisme), Pousses de plante (effets des radiations), Pousses de plante (métabolisme), Réaction de polymérisation en chaîne (MeSH), Techniques de culture de tissus (MeSH), Électrophorèse sur gel d'agar (MeSH).
- MESH :
- effets des radiations : Aérobiose, Photosynthèse, Populus, Pousses de plante.
- génétique : ADN des plantes.
- métabolisme : Bactéries, Dioxyde de carbone, Méthane, Populus, Pousses de plante.
- Isotopes du carbone, Lumière, Marquage isotopique, Réaction de polymérisation en chaîne, Techniques de culture de tissus, Électrophorèse sur gel d'agar.
English descriptors
- KwdEn :
- Aerobiosis (radiation effects), Bacteria (metabolism), Carbon Dioxide (metabolism), Carbon Isotopes (MeSH), DNA, Plant (genetics), Electrophoresis, Agar Gel (MeSH), Isotope Labeling (MeSH), Light (MeSH), Methane (metabolism), Photosynthesis (radiation effects), Plant Shoots (metabolism), Plant Shoots (radiation effects), Polymerase Chain Reaction (MeSH), Populus (metabolism), Populus (radiation effects), Tissue Culture Techniques (MeSH).
- MESH :
- chemical , genetics : DNA, Plant.
- chemical , metabolism : Carbon Dioxide, Methane.
- metabolism : Bacteria, Plant Shoots, Populus.
- radiation effects : Aerobiosis, Photosynthesis, Plant Shoots, Populus.
- chemical : Carbon Isotopes, Electrophoresis, Agar Gel, Isotope Labeling, Light, Polymerase Chain Reaction, Tissue Culture Techniques.
Abstract
The aerobic formation of methane in plants has been reported previously, but has been questioned by a number of researchers. Recently, isotopic evidence demonstrated that ultraviolet irradiation and heating lead to photochemical or thermal aerobic methane formation mainly from plant pectin in the absence of microbial methane production. However, the origin of aerobic methane formation from plant material observed under low temperature and low-light/dark conditions is still unclear. Here we show that Grey poplar (Populus × canescens, syn. Populus tremula × Populus alba) plants derived from cell cultures under sterile conditions released 13C-labeled methane under low-light conditions after feeding the plants with 13CO2. Molecular biological analysis proved the absence of any microbial contamination with known methanogenic microorganisms and ruled out the possibility that methane emission from our poplar shoot cultures under aerobic low-light/dark and ambient temperature conditions could be of microbial origin. The CH4 release rates in our experiment were in the range of 0.16-0.7 ng g-1 DW h-1, adding evidence to the growing opinion that the quantitative role of aerobic methane emissions from plants in the global methane budget, at least from cold temperate or boreal regions, is only of minor importance.
DOI: 10.1111/j.1469-8137.2009.02797.x
PubMed: 19281477
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.</title><author><name sortKey="Bruggemann, Nicolas" sort="Bruggemann, Nicolas" uniqKey="Bruggemann N" first="Nicolas" last="Brüggemann">Nicolas Brüggemann</name><affiliation wicri:level="1"><nlm:affiliation>Research Centre Karlsruhe, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Centre Karlsruhe, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen</wicri:regionArea><wicri:noRegion>82467 Garmisch-Partenkirchen</wicri:noRegion><wicri:noRegion>82467 Garmisch-Partenkirchen</wicri:noRegion><wicri:noRegion>D-82467 Garmisch-Partenkirchen</wicri:noRegion></affiliation></author><author><name sortKey="Meier, Rudolf" sort="Meier, Rudolf" uniqKey="Meier R" first="Rudolf" last="Meier">Rudolf Meier</name></author><author><name sortKey="Steigner, Dominik" sort="Steigner, Dominik" uniqKey="Steigner D" first="Dominik" last="Steigner">Dominik Steigner</name></author><author><name sortKey="Zimmer, Ina" sort="Zimmer, Ina" uniqKey="Zimmer I" first="Ina" last="Zimmer">Ina Zimmer</name></author><author><name sortKey="Louis, Sandrine" sort="Louis, Sandrine" uniqKey="Louis S" first="Sandrine" last="Louis">Sandrine Louis</name></author><author><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19281477</idno><idno type="pmid">19281477</idno><idno type="doi">10.1111/j.1469-8137.2009.02797.x</idno><idno type="wicri:Area/Main/Corpus">003621</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003621</idno><idno type="wicri:Area/Main/Curation">003621</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003621</idno><idno type="wicri:Area/Main/Exploration">003621</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.</title><author><name sortKey="Bruggemann, Nicolas" sort="Bruggemann, Nicolas" uniqKey="Bruggemann N" first="Nicolas" last="Brüggemann">Nicolas Brüggemann</name><affiliation wicri:level="1"><nlm:affiliation>Research Centre Karlsruhe, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Centre Karlsruhe, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen</wicri:regionArea><wicri:noRegion>82467 Garmisch-Partenkirchen</wicri:noRegion><wicri:noRegion>82467 Garmisch-Partenkirchen</wicri:noRegion><wicri:noRegion>D-82467 Garmisch-Partenkirchen</wicri:noRegion></affiliation></author><author><name sortKey="Meier, Rudolf" sort="Meier, Rudolf" uniqKey="Meier R" first="Rudolf" last="Meier">Rudolf Meier</name></author><author><name sortKey="Steigner, Dominik" sort="Steigner, Dominik" uniqKey="Steigner D" first="Dominik" last="Steigner">Dominik Steigner</name></author><author><name sortKey="Zimmer, Ina" sort="Zimmer, Ina" uniqKey="Zimmer I" first="Ina" last="Zimmer">Ina Zimmer</name></author><author><name sortKey="Louis, Sandrine" sort="Louis, Sandrine" uniqKey="Louis S" first="Sandrine" last="Louis">Sandrine Louis</name></author><author><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerobiosis (radiation effects)</term><term>Bacteria (metabolism)</term><term>Carbon Dioxide (metabolism)</term><term>Carbon Isotopes (MeSH)</term><term>DNA, Plant (genetics)</term><term>Electrophoresis, Agar Gel (MeSH)</term><term>Isotope Labeling (MeSH)</term><term>Light (MeSH)</term><term>Methane (metabolism)</term><term>Photosynthesis (radiation effects)</term><term>Plant Shoots (metabolism)</term><term>Plant Shoots (radiation effects)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (metabolism)</term><term>Populus (radiation effects)</term><term>Tissue Culture Techniques (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Aérobiose (effets des radiations)</term><term>Bactéries (métabolisme)</term><term>Dioxyde de carbone (métabolisme)</term><term>Isotopes du carbone (MeSH)</term><term>Lumière (MeSH)</term><term>Marquage isotopique (MeSH)</term><term>Méthane (métabolisme)</term><term>Photosynthèse (effets des radiations)</term><term>Populus (effets des radiations)</term><term>Populus (métabolisme)</term><term>Pousses de plante (effets des radiations)</term><term>Pousses de plante (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Techniques de culture de tissus (MeSH)</term><term>Électrophorèse sur gel d'agar (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Dioxide</term><term>Methane</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Aérobiose</term><term>Photosynthèse</term><term>Populus</term><term>Pousses de plante</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bactéries</term><term>Dioxyde de carbone</term><term>Méthane</term><term>Populus</term><term>Pousses de plante</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Aerobiosis</term><term>Photosynthesis</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Isotopes</term><term>Electrophoresis, Agar Gel</term><term>Isotope Labeling</term><term>Light</term><term>Polymerase Chain Reaction</term><term>Tissue Culture Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Isotopes du carbone</term><term>Lumière</term><term>Marquage isotopique</term><term>Réaction de polymérisation en chaîne</term><term>Techniques de culture de tissus</term><term>Électrophorèse sur gel d'agar</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aerobic formation of methane in plants has been reported previously, but has been questioned by a number of researchers. Recently, isotopic evidence demonstrated that ultraviolet irradiation and heating lead to photochemical or thermal aerobic methane formation mainly from plant pectin in the absence of microbial methane production. However, the origin of aerobic methane formation from plant material observed under low temperature and low-light/dark conditions is still unclear. Here we show that Grey poplar (Populus × canescens, syn. Populus tremula × Populus alba) plants derived from cell cultures under sterile conditions released 13C-labeled methane under low-light conditions after feeding the plants with 13CO2. Molecular biological analysis proved the absence of any microbial contamination with known methanogenic microorganisms and ruled out the possibility that methane emission from our poplar shoot cultures under aerobic low-light/dark and ambient temperature conditions could be of microbial origin. The CH4 release rates in our experiment were in the range of 0.16-0.7 ng g-1 DW h-1, adding evidence to the growing opinion that the quantitative role of aerobic methane emissions from plants in the global methane budget, at least from cold temperate or boreal regions, is only of minor importance.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19281477</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>182</Volume><Issue>4</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.</ArticleTitle><Pagination><MedlinePgn>912-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2009.02797.x</ELocationID><Abstract><AbstractText>The aerobic formation of methane in plants has been reported previously, but has been questioned by a number of researchers. Recently, isotopic evidence demonstrated that ultraviolet irradiation and heating lead to photochemical or thermal aerobic methane formation mainly from plant pectin in the absence of microbial methane production. However, the origin of aerobic methane formation from plant material observed under low temperature and low-light/dark conditions is still unclear. Here we show that Grey poplar (Populus × canescens, syn. Populus tremula × Populus alba) plants derived from cell cultures under sterile conditions released 13C-labeled methane under low-light conditions after feeding the plants with 13CO2. Molecular biological analysis proved the absence of any microbial contamination with known methanogenic microorganisms and ruled out the possibility that methane emission from our poplar shoot cultures under aerobic low-light/dark and ambient temperature conditions could be of microbial origin. The CH4 release rates in our experiment were in the range of 0.16-0.7 ng g-1 DW h-1, adding evidence to the growing opinion that the quantitative role of aerobic methane emissions from plants in the global methane budget, at least from cold temperate or boreal regions, is only of minor importance.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brüggemann</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Research Centre Karlsruhe, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meier</LastName><ForeName>Rudolf</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Steigner</LastName><ForeName>Dominik</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Zimmer</LastName><ForeName>Ina</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Louis</LastName><ForeName>Sandrine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Schnitzler</LastName><ForeName>Jörg-Peter</ForeName><Initials>JP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>OP0UW79H66</RegistryNumber><NameOfSubstance UI="D008697">Methane</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000332" MajorTopicYN="N">Aerobiosis</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004587" MajorTopicYN="N">Electrophoresis, Agar Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007553" MajorTopicYN="N">Isotope Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008027" MajorTopicYN="Y">Light</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008697" MajorTopicYN="N">Methane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046509" MajorTopicYN="Y">Tissue Culture Techniques</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>3</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>3</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>6</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19281477</ArticleId><ArticleId IdType="pii">NPH2797</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2009.02797.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Louis, Sandrine" sort="Louis, Sandrine" uniqKey="Louis S" first="Sandrine" last="Louis">Sandrine Louis</name><name sortKey="Meier, Rudolf" sort="Meier, Rudolf" uniqKey="Meier R" first="Rudolf" last="Meier">Rudolf Meier</name><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name><name sortKey="Steigner, Dominik" sort="Steigner, Dominik" uniqKey="Steigner D" first="Dominik" last="Steigner">Dominik Steigner</name><name sortKey="Zimmer, Ina" sort="Zimmer, Ina" uniqKey="Zimmer I" first="Ina" last="Zimmer">Ina Zimmer</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Bruggemann, Nicolas" sort="Bruggemann, Nicolas" uniqKey="Bruggemann N" first="Nicolas" last="Brüggemann">Nicolas Brüggemann</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003549 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003549 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19281477
|texte= Nonmicrobial aerobic methane emission from poplar shoot cultures under low-light conditions.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19281477" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |